Can I read A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas on EtoBox?

A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas by Hongxia Guan; Panpan Xue; Haijian Zhou; Dan Sha; Duochun Wang; He Gao; Jie Li; Baowei Diao; Hongqun Zhao; Biao Kan; Jingyun Zhang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas about?

A multiplex PCR (mPCR) assay was established to detect five pathogenic Vibrio species and Plesiomonas shigelloides. Twelve genes were included: ompW, ctxA, rfbN, and wbfR from V. cholerae; tl, tdh, and trh from V. parahaemolyticus; toxR and vmhA from V. mimicus; toxR from V. fluvialis; vvhA from V. vulnificus; and the 23S rRNA gene from P. shigelloides. The specificity of the mPCR assay was 100% for the detection of 136 strains and the limits of detection (LoD) were 12.5-50 pg/reaction. The assay exhibited higher sensitivity than cultivation methods in the detection of APW cultures of 113 diarrhea samples. In the analysis of 369 suspected Vibrio populations from estuarine water samples, the specificity of the mPCR for V. cholerae and V. parahaemolyticus was 100% for both, while the sensitivities were 100% and 96.1%, respectively. The assay can be applied to screen enrichment cultures and suspected colonies from environmental and clinical samples.

Who reads A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas?

Author

Hongxia Guan; Panpan Xue; Haijian Zhou; Dan Sha; Duochun Wang; He Gao; Jie Li; Baowei Diao; Hongqun Zhao; Biao Kan; Jingyun Zhang

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0890-8508)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)