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Identification of Basic Fibroblast Growth Factor Sensitivity and Receptor and Ligand Expression in Human Colon Tumor Cell Lines by Bonnie A. New; Lynn C. Yeoman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification of Basic Fibroblast Growth Factor Sensitivity and Receptor and Ligand Expression in Human Colon Tumor Cell Lines about?

Basic fibroblast growth factor (bFGF) has been shown to be mitogenic to many different eukaryotic cell lines of mesodermal and neuroectoderrnal origin. Addition of exogenous bFCF to the chemically defined media of five characterized human colon tumor cell lines, cultured in the absence of epidermal growth factor (EGF), resulted in stimulation of growth from 24% to 146% in four of five cell lines, as measured by a colorimetric MTT assay. A positive dose-response relationship was observed when colon cells were treated with bFGF concentrations from 1 p M to 1 nM. bFGF showed a cumulative effect with EGF in stimulating the proliferation of colon tumor cells. The growth-inhibitory effect of exogenous transforming growth factor-beta (TGF-P) on these cells was abolished by bFGF. When colon tumor cells were examined on immunoblots with a fibroblast growth factor (FGF) receptor-specific antibody, bands were detected at apparent molecular weights of 131 and 145 kDa. Conditioned media and cell lysates from the same human colon tumor cell lines were immunoprecipitated with a bFGF-specific antibody. An immunoreactive band was detected that comigrated with authentic human recombinant bFGF (1 6 k

Who reads Identification of Basic Fibroblast Growth Factor Sensitivity and Receptor and Ligand Expression in Human Colon Tumor Cell Lines?

Author

Bonnie A. New; Lynn C. Yeoman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)