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The effect of 17β-estradiol on IL-6 secretion and NF-κB DNA-binding activity in human retinal pigment epithelial cells by Tuomas Paimela; Tuomas Ryhänen; Eliisa Mannermaa; Johanna Ojala; Giedrius Kalesnykas; Antero Salminen; Kai Kaarniranta is a Immunology and Microbiology article available to read on EtoBox.

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Toll-like receptors (TLRs) and inflammatory cascades participate in the pathology of age-related macular degeneration (AMD). The effect of estrogens on the development of AMD is poorly understood, although many studies indicate that these compounds can modulate inflammatory responses. In this study, we investigated the regulatory role of TLR agonists and 17beta-estradiol (E(2)) on IL-6 expression and NF-kappaB DNA-binding activity in human retinal pigment epithelial cells (ARPE-19). The inflammatory response of ARPE-19 cells to various TLR agonists, e.g. Pam, zymosan, flagellin, SLTA and lipopolysaccharide (LPS) exposures were examined via the secretion of IL-6 cytokine as analyzed by ELISA. In addition, the IL-6 responses to the estrogen-receptor agonist, E(2), and to the estrogen-receptor antagonist ICI 182.780 as well as to the NF-kappaB inhibitor helenalin were compared. The DNA-binding activity of NF-kappaB transcription factor of nuclear cell extracts was analyzed by the gel mobility shift assay (EMSA). TLR4 gene expression was studied by quantitave PCR. The TLR4 agonist, LPS, caused a clear IL-6 response that was attenuated by E(2) in ARPE-19-cells. The anti-inflammatory pro

Who reads The effect of 17β-estradiol on IL-6 secretion and NF-κB DNA-binding activity in human retinal pigment epithelial cells?

Author

Tuomas Paimela; Tuomas Ryhänen; Eliisa Mannermaa; Johanna Ojala; Giedrius Kalesnykas; Antero Salminen; Kai Kaarniranta

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

2007

Language

EN

Field

Immunology and Microbiology (Life Sciences)