About this Biochemistry, Genetics and Molecular Biology article

Reversed-phase HPLC determination of chlorophyll a′ and phylloquinone in Photosystem I of oxygenic photosynthetic organisms : Universal existence of one chlorophyll a′ molecule in Photosystem I by Akimasa Nakamura; Masahiko Akai; Emi Yoshida; Takashi Taki; Tadashi Watanabe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Chlorophyll (Chl) __a__′, the C13^2^‐epimer of Chl __a__, is a constituent of the primary electron donor (P700) of Photosystem (PS) I of a thermophilic cyanobacterium __Synechococcus__ (__Thermosynechococcus__) __elongatus__, as was recently demonstrated by X‐ray crystallography. To determine whether PS I of oxygenic photosynthetic organisms universally contains one molecule of Chl __a__′, pigment compositions of thylakoid membranes and PS I complexes isolated from the cyanobacteria __T. elongatus__ and __Synechocystis__ sp. PCC 6803, the green alga __Chlamydomonas reinhardtii__, and the green plant spinach, were examined by simultaneous detection of phylloquinone (the secondary electron acceptor of PS I) and Chl __a__′ by reversed‐phase HPLC. The results were compared with the Chl __a__/P700 ratio determined spectrophotometrically. The Chl __a__′/PS I ratios of thylakoid membranes and PS I were about 1 for all the organisms examined, and one Chl __a__′ molecule was found in PS I even after most of the peripheral subunits were removed. Chl __a__′ showed a characteristic extraction behaviour significantly different from the bulk Chl __a__ in acetone/methanol extraction upon varying

Author

Akimasa Nakamura; Masahiko Akai; Emi Yoshida; Takashi Taki; Tadashi Watanabe

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)