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Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons by Diego M. Gelman; Daniela Noaín; M. Elena Avale; Verónica Otero; Malcolm J. Low; Marcelo Rubinstein is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Summary: To introduce restricted DNA recombination events into catecholaminergic neurons using the __Cre/loxP__ technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH‐positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH‐Cre mice with the floxed reporter strain Z/AP and observed efficient Cre‐mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of “floxed” genes in catecholaminergic neurons. genesis 36:196–202, 2003. © 2003 Wiley‐Liss, Inc.

Who reads Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons?

Author

Diego M. Gelman; Daniela Noaín; M. Elena Avale; Verónica Otero; Malcolm J. Low; Marcelo Rubinstein

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-954X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)