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High Performance Liquid Chromatography Equipped with a Cathodic Detector and Column-switching Device as a High-throughput Method for a Phosphatase Assay with P-nitrophenyl Phosphate by Yuji Yamauchi; Megumi Ido; Hatsuo Maeda is a Chemistry article available to read on EtoBox.

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LC coupled to an electrochemical detector (LC-EC) operating in cathodic mode with a column-switching system realizes a high-throughput detection of p-nitrophenol (NP). The measurement-time for each NP sample was shortened to 20 s, and the successive analyses of 39 samples was completed within 13 min. In the present system, the limits of detection and quantification were 0.15 and 0.20 M, respectively, and further, up to 25 M, a linear calibration curve was afforded. Relative standard deviations for standard solutions of 0.20, 1.0, and 25 M NP were 4.3, 2.0, and 1.1% (n = 5), respectively. Between-run precisions of the analysis of 5.0 and 25 M NP over 6 days were 4.8 and 1.3%, respectively. A comparison with the commonly used Bessey-Lowry-Brock method indicates that the present LC-EC is useful for the high-throughput assay of acid and alkaline phosphatases in urine and blood samples with a p-nitrophenyl phosphate substrate.

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Author

Yuji Yamauchi; Megumi Ido; Hatsuo Maeda

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)