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Can I read Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis on EtoBox?
Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis by Steward, Katherine F.; Eilers, Brian; Tripet, Brian; Fuchs, Amanda; Dorle, Michael; Rawle, Rachel; Soriano, Berliza; Balasubramanian, Narayanaganesh; Copié, Valérie; Bothner, Brian; Hatzenpichler, Roland is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis about?
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms. A basic premise of BONCAT is that the non-canonical amino acids (NCAA) used to track translational activity do not significantly alter cellular physiology. If the NCAA would induce changes in the metabolic state of cells, interpretation of BONCAT studies could be challenging. To address this knowledge-gap, we have used a global metabolomics analyses to assess the intracellular effects of NCAA incorporation. Two NCAA were tested: __L__-azidohomoalanine (AHA) and __L__-homopropargylglycine (HPG); __L__-methionine (MET) was used as a minimal stress baseline control. Liquid Chromatography Mass Spectrometry (LC-MS) and Nuclear Magnetic Resonance (NMR) were used to characterize intracellular metabolite profiles of __Escherichia coli__ cultures, with multivariate statistical analysis using XCMS and MetaboAnalyst. Results show that doping with NCAA induces metabolic changes, however, the metabolic impact was not dramatic. A second set of experiments in which cultures were placed under mild stress to simulate real-worl
Who reads Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Steward, Katherine F.; Eilers, Brian; Tripet, Brian; Fuchs, Amanda; Dorle, Michael; Rawle, Rachel; Soriano, Berliza; Balasubramanian, Narayanaganesh; Copié, Valérie; Bothner, Brian; Hatzenpichler, Roland
- Publisher
- Frontiers; Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2010-; Publons (ISSN 1664-302X)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)