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Analytical Strategies to Profile the Internal Chemical Exposome and the Metabolome of Human Placenta by Raghad Al-Salhi; Christine Monfort; Nathalie Bonvallot; Arthur David is a Chemistry article available to read on EtoBox.

What is Analytical Strategies to Profile the Internal Chemical Exposome and the Metabolome of Human Placenta about?

As a non-invasive biological matrix, the placenta offers great and novel opportunities to monitor fetal exposure to exogenous chemicals and their biotransformation products (the internal chemical exposome), as well as the biological responses associated, in large-scale epidemiological studies. However, it is first crucial to ensure that analytical methods based on high-resolution mass spectrometry (HRMS) can detect the low abundant components of the internal chemical exposome present in these complex biological matrices. In this study, we aimed to develop a robust analytical method (extraction and sample preparation) sensitive enough to profile the internal chemical exposome and the metabolome of placenta using high-resolution mass spectrometry (HRMS) for future application in mother child cohorts. Several extraction solvents (methanol, methanol/H2O (50/50 v/v), methyl tert-butyl ether/methanol/H2O) were tested and their ability to extract components of the internal exposome and metabolome were compared. Then, sample preparation methods commonly used for metabolomics application (methanolic protein precipitation) were compared with solid phase extraction (SPE), protein and phosphol

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Author

Raghad Al-Salhi; Christine Monfort; Nathalie Bonvallot; Arthur David

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Chemistry (Physical Sciences)