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Can I read Dietary flavonoid carvacrol triggers the apoptosis of human breast cancer MCF-7 cells via the p53/Bax/Bcl-2 axis on EtoBox?
Dietary flavonoid carvacrol triggers the apoptosis of human breast cancer MCF-7 cells via the p53/Bax/Bcl-2 axis by Ayat Moradipour; Hassan Dariushnejad; Changiz Ahmadizadeh; Hamed Esmaiil Lashgarian is a Medicine article available to read on EtoBox.
What is Dietary flavonoid carvacrol triggers the apoptosis of human breast cancer MCF-7 cells via the p53/Bax/Bcl-2 axis about?
Recently, numerous studies have shown that natural compounds such as carvacrol can be used as therapeutic agents for medicinal purposes. Although carvacrol was found to suppress breast cancer cell lines, however, whether the therapeutic effect of carvacrol on breast cancer is caused by increasing the expression of pro-apoptotic genes in the MCF-7 cell line has not been studied. In this research, we investigate the effect of carvacrol on the expression of P53, pro-apoptotic Bax and anti-apoptotic BCL-2 genes in MCF-7 cells. After preparation and cultivation of MCF-7 cells, the IC50 value of carvacrol on the cells was evaluated by MTT assay, and then apoptosis induction was observed in the cell line treated with different concentrations of carvacrol by DAPI staining. To assess the expression level of Bax, P53 and Bcl-2 both in genes and protein levels QPCR method and western blot analysis were used. According to the results of the research, it was determined that the IC50 of carvacrol compound in MCF-7 cells is 305 μM. DAPI staining exhibit apoptosis and morphological changes in treated MCF-7 cells. Real-time PCR assay and western blot showed increasing the Bax and P53 expression and
Who reads Dietary flavonoid carvacrol triggers the apoptosis of human breast cancer MCF-7 cells via the p53/Bax/Bcl-2 axis?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Ayat Moradipour; Hassan Dariushnejad; Changiz Ahmadizadeh; Hamed Esmaiil Lashgarian
- Publisher
- Springer Science and Business Media LLC
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)