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A recombinant cytotoxic chimera based on mammalian deoxyribonuclease-I by Helena Linardou; Agamemnon A. Epenetos; Mahendra P. Deonarain is a Medicine article available to read on EtoBox.
What is A recombinant cytotoxic chimera based on mammalian deoxyribonuclease-I about?
A number of mammalian proteins with suitable biological activities have been considered for use in targeted tumour therapy. Deoxyribonuclease-I (DNase-I), an endonuclease that degrades double-stranded DNA, represents an attractive candidate for tumour targeting since it is normally non-toxic yet could be highly cytotoxic when redirected to the cell nucleus. Our aim was to investigate the cytotoxic potential of mammalian DNase-I and its possible use in tumour-targeting strategies for cancer therapy. A chimeric molecule comprising a scFv reactive against the human placental alkaline phosphatase (hPLAP) and bovine pancreatic DNase-I was designed and investigated. The development of a tightly controlled system for the bacterial expression of DNase-I and its chimera is described. The production and purification of active DNase-I from the soluble cell fraction and significant yields from the insoluble fraction by isolation and refolding are described. The construction, expression, purification and in vitro characterisation of an anti-PLAP scFv-DNase-I chimera is also described. This molecule was shown to possess both antigen-binding and DNA-degrading activity in in vitro assays, thus com
Who reads A recombinant cytotoxic chimera based on mammalian deoxyribonuclease-I?
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- Author
- Helena Linardou; Agamemnon A. Epenetos; Mahendra P. Deonarain
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2000
- Language
- EN
- Field
- Medicine (Health Sciences)