About this Biochemistry, Genetics and Molecular Biology article

Targeting STAT3 enhances NDV‐induced immunogenic cell death in prostate cancer cells by Xueke Wang; Xiaoyan Shao; Linaer Gu; Ke Jiang; Sitong Wang; Jianhua Chen; Juemin Fang; Xianling Guo; Min Yuan; Ji Shi; Chan Ding; Songshu Meng; Qing Xu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract Oncolytic Newcastle disease virus (NDV) induces immunogenic cell death (ICD), liberating danger‐associated molecular patterns (DAMPs) that provokes defiance in neoplastic malignancy. The present study aims to investigate whether and how oncolytic NDV triggers ICD in prostate cancer cells. We show that NDV/FMW, an oncolytic NDV strain FMW, elicited the expression and release of several ICD markers, that is calreticulin (CRT), heat shock proteins (HSP70/90) and high‐mobility group box 1 (HMGB1), in prostate cancer cells. Furthermore, pharmacological repression of apoptosis, necroptosis, autophagy or endoplasmic reticulum (ER) stress exerted diverse effects on the HMGB1 and HSP70/90 evacuation in NDV/FMW‐infected prostate cancer cells. Moreover, ICD markers induced in prostate cancer cells upon NDV/FMW infection, were enhanced by either treatment with a STAT3 (signal transducer and activator of transcription 3) inhibitor or shRNA‐mediated knockdown of STAT3. In nude mice bearing prostate cancer cell‐derived tumours, the tumours injected with the supernatants of NDV/FMW‐infected cells grew smaller than mock‐treated tumours. These results indicate that oncolytic NDV provokes

Author

Xueke Wang; Xiaoyan Shao; Linaer Gu; Ke Jiang; Sitong Wang; Jianhua Chen; Juemin Fang; Xianling Guo; Min Yuan; Ji Shi; Chan Ding; Songshu Meng; Qing Xu

Publisher

Carol Davila University of Medicine Bucharest, Romania; Wiley (Blackwell Publishing); Wiley-Blackwell; Wiley (ISSN 1582-1838)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)