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Cluster analysis of selected Mycobacterium species using rifampicin resistance DNA-directed RNA polymerase beta subunit (rpoB) gene by O.S. Amoo; I. Taiwo; F. Anwoju is a Medicine article available to read on EtoBox.

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Background: Tuberculosis (TB) is a major global health threat, aggravated by emergence of drug resistance. Early diagnosis is critical and has been offered lately by molecular assays. Aim: This study aimed to evaluate the performance of GeneXpert ® MTB/RIF and Anyplex TM II MTB/MDR assays in simultaneous direct detection of Mycobacterium tuberculosis (MTB) and genetic determination of Rifampicin (RF) resistance. Methods and materials: This study was conducted with 100 specimens collected from TB suspects. ZN smears were prepared and L-J culture was used for isolation of MTB. GeneXpert and Anyplex assays were performed directly on specimens to detect Mycobacterium tuberculosis (MTB) and RF resistance. Results: MTB was isolated on L-J culture from 40% of specimens. For MTB detection, Xpert and Anyplex assays showed same total sensitivity of 95%, and specificities of 73.3% and 81.7%, respectively. RF resistance was detected by Xpert and Anyplex assays with rates of 5.5% and 4.08%, respectively showing significant agreement with a P-value < 0.001. Time of assay was 2 h for Xpert and 3 h for Anyplex. Conclusion: Both molecular assays enable rapid concomitant detection of MTB and drug re

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Author

O.S. Amoo; I. Taiwo; F. Anwoju

Publisher

Elsevier BV

Published

2020

Language

EN

Field

Medicine (Health Sciences)