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In Vivo Use of Oligonucleotides to Inhibit Choroidal Neovascularisation in the Eye by Kerryn L. Garrett; Wei-Yong Shen; Piroska E. Rakoczy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is In Vivo Use of Oligonucleotides to Inhibit Choroidal Neovascularisation in the Eye about?

Background We have previously demonstrated the in vivo uptake of oligonucleotides in the rat eye and have continued with experiments to look at the effectiveness of targeted oligonucleotide sequences. Vascular endothelial growth factor (VEGF) is correlated with new blood vessel formation and has been implicated in numerous eye diseases characterised by abnormal blood vessel proliferation. An oligonucleotide targeted to the VEGF sequence was examined for its effect on VEGF production in vitro and the development of choroidal neovascularisation in vivo in the eye. Methods A series of sequences were assessed in an in vitro screening system using retinal pigment epithelial (RPE) cells to demonstrate a reduction in VEGF. A targeted sequence was further investigated using an animal model of choroidal neovascularisation where a krypton laser was used to produce a wound healing response in the choroid and retina. The oligonucleotide was injected into the vitreous and the development of choroidal neovascularisation assessed using fluorescein angiography. ## Results The targeted sequence was shown in vitro to downregulate the VEGF produced by RPE cells grown under hypoxic conditions and when

Who reads In Vivo Use of Oligonucleotides to Inhibit Choroidal Neovascularisation in the Eye?

Author

Kerryn L. Garrett; Wei-Yong Shen; Piroska E. Rakoczy

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)