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Allopregnanolone Serum Levels and Expression of 5α-Reductase and 3α-Hydroxysteroid Dehydrogenase Isoforms in Hippocampal and Temporal Cortex of Patients with Epilepsy by Birgit Stoffel-Wagner; Matthias Watzka; Stephan Steckelbroeck; Michael Ludwig; Hans Clusmann; Frank Bidlingmaier; Elena Casarosa; Stefano Luisi; Christian E Elger; Stefan Beyenburg is a Medicine article available to read on EtoBox.
What is Allopregnanolone Serum Levels and Expression of 5α-Reductase and 3α-Hydroxysteroid Dehydrogenase Isoforms in Hippocampal and Temporal Cortex of Patients with Epilepsy about?
In the human central nervous system, progesterone is rapidly metabolised to 5 alpha-dihydroprogesterone which subsequently is further reduced to allopregnanolone (AP). These conversions are catalysed by 5 alpha-reductase and 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD). Although different isoforms of both enzymes have been identified in the brain, our knowledge of their expression in the human brain remains limited. The aim of the present study was to investigate the mRNA expression of 5 alpha-reductase 1 as well as 3 alpha-HSD 1, 2, 3 and 20 alpha-HSD in brain tissue from patients with pharmacoresistant temporal lobe epilepsy (TLE). Specimens were derived from either the hippocampus or the temporal lobe cortex and from the tumor-free approach corridor tissue of patients with brain tumors. Quantification of different mRNAs was achieved by real time PCR. In addition, we provide data on simultaneous evaluation of serum AP concentrations. We could demonstrate that 3 alpha-HSD 1 was not expressed in the hippocampus and temporal lobe of patients with TLE. In the hippocampus and temporal lobe, the expression levels of 3 alpha-HSD 2 were about 20% of that in liver tissue, those of 3
Who reads Allopregnanolone Serum Levels and Expression of 5α-Reductase and 3α-Hydroxysteroid Dehydrogenase Isoforms in Hippocampal and Temporal Cortex of Patients with Epilepsy?
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- Author
- Birgit Stoffel-Wagner; Matthias Watzka; Stephan Steckelbroeck; Michael Ludwig; Hans Clusmann; Frank Bidlingmaier; Elena Casarosa; Stefano Luisi; Christian E Elger; Stefan Beyenburg
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0920-1211)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)