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Can I read Enhancements in Gene Expression by the Choice of Plasmid DNA Formulations Containing Neutral Polymeric Excipients on EtoBox?
Enhancements in Gene Expression by the Choice of Plasmid DNA Formulations Containing Neutral Polymeric Excipients by Chin-Yi Huang; San San Ma; Sandra Lee; Ramachandran Radhakrishnan; Chad S. Braun; Sirirat Choosakoonkriang; Chris M. Wiethoff; Brian A. Lobo; C. Russell Middaugh is a Chemistry article available to read on EtoBox.
What is Enhancements in Gene Expression by the Choice of Plasmid DNA Formulations Containing Neutral Polymeric Excipients about?
Formulations containing maltodextrin (2% w/v) were identified to facilitate intramuscular (im) delivery of plasmid DNA in mice using the reporter genes luciferase and chloramphenicol acetyltransferase (CAT) and the therapeutic gene of erythropoietin (EPO) as monitors of transfection efficiency. Even though considerable variability in gene expression was observed in animals, a 5-8-fold enhancement of reporter gene expression was observed with this excipient compared with saline formulations of DNA. In a therapeutically significant experiment, a single im injection of an EPO plasmid formulation containing 2% (w/v) maltodextrin resulted in a significant and prolonged elevation of the hematocrit levels of mice compared with control DNA in saline. Biophysical studies with Fourier transform infrared (FTIR) spectroscopy, isothermal titration, and differential scanning calorimetry (DSC) suggested a weak interaction between DNA and maltodextrin as well as a thermal stabilizing effect on the DNA. These in vivo and biophysical results with maltodextrin are comparable to those reported previously with other nonionic polymers, such as poly(vinyl pyrrolidone) and poloxamers, and indicate that ma
Who reads Enhancements in Gene Expression by the Choice of Plasmid DNA Formulations Containing Neutral Polymeric Excipients?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Chin-Yi Huang; San San Ma; Sandra Lee; Ramachandran Radhakrishnan; Chad S. Braun; Sirirat Choosakoonkriang; Chris M. Wiethoff; Brian A. Lobo; C. Russell Middaugh
- Publisher
- John Wiley and Sons; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0022-3549)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)