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Development of an ethidium monoazide enhanced internally controlled universal 16S rDNA by Poorvi Patel, Jeremy A. Garson, Kate I. Tettmar, Siobhan Ancliff, Carl McDonald, Tyrone Pitt,Juliana Coelho, and Richard S. Tedder is a book available to read on EtoBox.

What is Development of an ethidium monoazide enhanced internally controlled universal 16S rDNA about?

na Coelho, and Richard S. TedderBACKGROUND: Bacterial contamination of plateletWhile the risk of transfusion-transmitted viralinfections has been reduced dramatically(PLT) concentrates remains a problem for blood transover the past few decades, the problem offusion services. Culture-based bacterial screening techbacterial contamination has proved moreniques are available but offer inadequate speed anddifficult to resolve. Although transfusion-transmitted bacsensitivity. Alternative techniques based on polymeraseterial infections have been reduced in recent years throughchain reaction (PCR) amplification have been describeduse of improved skin cleansing and diversion methods,but their performance is often compromised by traces offurther work is required to eliminate the problem.1 The riskbacterial DNA in reagents.of bacterial contamination is greatest for platelet (PLT) conSTUDY DESIGN AND METHODS: Universal 16S rDNAcentrates which, to preserve PLT function, are stored at 22primers were used to develop a real-time PCR assayto 24°C with constant agitation. These conditions provide(TaqMan, Applied Biosystems) and various reagentcontaminating bacteria with an excellent growth envir

Author

Poorvi Patel, Jeremy A. Garson, Kate I. Tettmar, Siobhan Ancliff, Carl McDonald, Tyrone Pitt,Juliana Coelho, and Richard S. Tedder

Published

2012

Language

EN