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EcoRI is a restriction endonuclease enzyme derived from Escherichia coli RY13, which recognizes and cuts the DNA sequence GAATTC. The resulting sticky ends facilitate the joining of foreign DNA with vector DNA through hydrogen bonding and the action of DNA ligase. Plasmids, which can replicate independently within bacterial cells, are used as vectors in gene cloning due to their high copy number and essential features like ori sites and selectable markers.

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rajendranpachaiyammal

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