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Mutational Analysis of a Plant Heat Shock Element by M. Dulce Barros; Eva Czarnecka; William B. Gurley is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Mutational Analysis of a Plant Heat Shock Element about?

A total of 32 mutations were generated within the TATA-proximal site 1 ( -72 to -47) of soybean heat shock gene GmhspI7.5E in order to functionally define the optimal configuration of sequences within the heat shock element (HSE). Mutants were tested in vivo utilizing sunflower tumors transformed by a T-DNA based vector. Promoter activity was determined by S 1 nuclease hybrid protection analysis of tumor transcripts. A total of five repeats (5'-nGAAn-3' or 5'-nTTCn-3') which comprise the H S E at site 1 were required for full transcription induction by heat stress. Analysis of non-conserved bases flanking the central trinucleotide block indicated that 5'-aGAAg'-3' is the optimum sequence for the 5 bp repeat.

Who reads Mutational Analysis of a Plant Heat Shock Element?

Author

M. Dulce Barros; Eva Czarnecka; William B. Gurley

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1992

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)