Skip to content

Opening book details…

Can I read SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface on EtoBox?

SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface by Bertoglio, Federico (author);Meier, Doris (author);Langreder, Nora (author);Steinke, Stephan (author);Rand, Ulfert (author);Simonelli, Luca (author);Heine, Philip Alexander (author);Ballmann, Rico (author);Schneider, Kai-Thomas (author);Roth, Kristian Daniel Ralph (author);Ruschig, Maximilian (author);Riese, Peggy (author);Eschke, Kathrin (author);Kim, Yeonsu (author);Schäckermann, Dorina (author);Pedotti, Mattia (author);Kuhn, Philipp (author);Zock-Emmenthal, Susanne (author);Wöhrle, Johannes (author);Kilb, Normann (author);Herz, Tobias (author);Becker, Marlies (author);Grasshoff, Martina (author);Wenzel, Esther Veronika (author);Russo, Giulio (author);Kröger, Andrea (author);Brunotte, Linda (author);Ludwig, Stephan (author);Fühner, Viola (author);Krämer, Stefan Daniel (author);Dübel, Stefan (author);Varani, Luca (author);Roth, Günter (author);Čičin-Šain, Luka (author);Schubert, Maren (author);Hust, Michael (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface about?

## Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, a new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with the receptor binding domain (RBD) of the S1 subunit of the viral spike protein. We present the use of phage display to select anti-SARS-CoV-2 spike antibodies from the human naïve antibody gene libraries HAL9/10 and subsequent identification of 309 unique fully human antibodies against S1. 17 antibodies are binding to the RBD, showing inhibition of spike binding to cells expressing ACE2 as scFv-Fc and neutralize active SARS-CoV-2 virus infection of VeroE6 cells. The antibody STE73-2E9 is showing neutralization of active SARS-CoV-2 as IgG and is binding to the ACE2-RBD interface. Thus, universal libraries from healthy human donors offer the advantage that antibodies can be generated quickly and independent from the availability of material from recovering patients in a pandemic situation.

Who reads SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Bertoglio, Federico (author);Meier, Doris (author);Langreder, Nora (author);Steinke, Stephan (author);Rand, Ulfert (author);Simonelli, Luca (author);Heine, Philip Alexander (author);Ballmann, Rico (author);Schneider, Kai-Thomas (author);Roth, Kristian Daniel Ralph (author);Ruschig, Maximilian (author);Riese, Peggy (author);Eschke, Kathrin (author);Kim, Yeonsu (author);Schäckermann, Dorina (author);Pedotti, Mattia (author);Kuhn, Philipp (author);Zock-Emmenthal, Susanne (author);Wöhrle, Johannes (author);Kilb, Normann (author);Herz, Tobias (author);Becker, Marlies (author);Grasshoff, Martina (author);Wenzel, Esther Veronika (author);Russo, Giulio (author);Kröger, Andrea (author);Brunotte, Linda (author);Ludwig, Stephan (author);Fühner, Viola (author);Krämer, Stefan Daniel (author);Dübel, Stefan (author);Varani, Luca (author);Roth, Günter (author);Čičin-Šain, Luka (author);Schubert, Maren (author);Hust, Michael (author)
Publisher
Springer Science and Business Media LLC
Published
2021
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

More by Bertoglio, Federico (author);Meier, Doris (author);Langreder, Nora (author);Steinke, Stephan (author);Rand, Ulfert (author);Simonelli, Luca (author);Heine, Philip Alexander (author);Ballmann, Rico (author);Schneider, Kai-Thomas (author);Roth, Kristian Daniel Ralph (author);Ruschig, Maximilian (author);Riese, Peggy (author);Eschke, Kathrin (author);Kim, Yeonsu (author);Schäckermann, Dorina (author);Pedotti, Mattia (author);Kuhn, Philipp (author);Zock-Emmenthal, Susanne (author);Wöhrle, Johannes (author);Kilb, Normann (author);Herz, Tobias (author);Becker, Marlies (author);Grasshoff, Martina (author);Wenzel, Esther Veronika (author);Russo, Giulio (author);Kröger, Andrea (author);Brunotte, Linda (author);Ludwig, Stephan (author);Fühner, Viola (author);Krämer, Stefan Daniel (author);Dübel, Stefan (author);Varani, Luca (author);Roth, Günter (author);Čičin-Šain, Luka (author);Schubert, Maren (author);Hust, Michael (author)

Browse all works by Bertoglio, Federico (author);Meier, Doris (author);Langreder, Nora (author);Steinke, Stephan (author);Rand, Ulfert (author);Simonelli, Luca (author);Heine, Philip Alexander (author);Ballmann, Rico (author);Schneider, Kai-Thomas (author);Roth, Kristian Daniel Ralph (author);Ruschig, Maximilian (author);Riese, Peggy (author);Eschke, Kathrin (author);Kim, Yeonsu (author);Schäckermann, Dorina (author);Pedotti, Mattia (author);Kuhn, Philipp (author);Zock-Emmenthal, Susanne (author);Wöhrle, Johannes (author);Kilb, Normann (author);Herz, Tobias (author);Becker, Marlies (author);Grasshoff, Martina (author);Wenzel, Esther Veronika (author);Russo, Giulio (author);Kröger, Andrea (author);Brunotte, Linda (author);Ludwig, Stephan (author);Fühner, Viola (author);Krämer, Stefan Daniel (author);Dübel, Stefan (author);Varani, Luca (author);Roth, Günter (author);Čičin-Šain, Luka (author);Schubert, Maren (author);Hust, Michael (author)