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This document summarizes a study on detecting Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight in rice, using a polyclonal antibody (PAb) and an enzyme-linked immunosorbent assay (ELISA) technique. PAb derived from an Indonesian Xoo isolate showed positive reactions against all Xoo isolates tested. The minimum detectable concentration of Xoo antigen was approximately 104 cells/ml. The PAb detected Xoo antigen in crude extracts, heated cells, and pure cultures of Xoo samples from ric
- Author
- Ngaji Alip
- Language
- EN