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Analysis of HIC-1 methylation and transcription in human ependymomas by Anke Waha; Arend Koch; Wolfgang Hartmann; Heike Mack; Johannes Schramm; Niels Sörensen; Frank Berthold; Otmar D. Wiestler; Torsten Pietsch; Andreas Waha is a Medicine article available to read on EtoBox.
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## Abstract Ependymomas are among the most common brain tumors in children. They develop from ependymal cells lining the ventricular system of the CNS. Previous studies have demonstrated a significant rate of allelic loss at chromosome 17p13.3. The __HIC‐1__ putative tumor‐suppressor gene, which exhibits hypermethylation and loss of expression in various tumor entities including medulloblastomas and gliomas, maps to the affected region. In the present study, we analyzed __HIC‐1__ in ependymomas. Therefore, we applied methylation‐specific PCR of the 5′‐untranslated region as well as of a central region of __HIC‐1__ and bisulfite sequencing to determine the methylation status in 52 ependymomas of different histologic subtypes, grades and locations. In addition, we used a competitive RT‐PCR approach for sensitive assessment of __HIC‐1__ transcripts. Hypermethylation of at least one of the 2 analyzed regions was found in 43/52 (83%) cases. There was a significant correlation between hypermethylation of __HIC‐1__ and nonspinal localization (__p__ = 0.019) as well as age. Of 27 ependymomas, 22 (81%) showed absent or low expression of __HIC‐1__. The elevated methylation of __HIC‐1__ in no
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- Author
- Anke Waha; Arend Koch; Wolfgang Hartmann; Heike Mack; Johannes Schramm; Niels Sörensen; Frank Berthold; Otmar D. Wiestler; Torsten Pietsch; Andreas Waha
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)