Can I read Klotho Protects Against Aged Myocardial Cells by Attenuating Ferroptosis on EtoBox?

Klotho Protects Against Aged Myocardial Cells by Attenuating Ferroptosis by Xicheng Xiong; Gang Wang; Yiping Wang; Tian Zhang; Yali Bao; Kun Wang; Dina Ainiwaer; Zhan Sun is a scholarly article available to read on EtoBox.

What is Klotho Protects Against Aged Myocardial Cells by Attenuating Ferroptosis about?

Klotho (KL) is a renal protein with aging-suppression properties that mediates its regulatory effect during cardiac fibroblast aging. However, to determine whether KL can protect aged myocardial cells by attenuating ferroptosis, this study aimed to investigate the protective effect of KL on aged cells and to explore its potential mechanism. Cell injury of H9C2 cells was induced with D-galactose (D-gal) and treated with KL in vitro. This study demonstrated that D-gal induces aging in H9C2 cells. D-gal treatment increased β-GAL(β-galactosidase) activity, decreased cell viability, enhanced oxidative stress, reduced mitochondrial cristae, and decreased the expression of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase-4 (GPx4), and P53, which are primary regulators of ferroptosis. The results showed that KL can eliminate D-gal-induced aging in H9C2 cells, likely due to its ability to increase the expression of the ferroptosis-associated proteins SLC7A11 and GPx4. Moreover, pifithrin-α, a P53-specific inhibitor, enhanced the expression of SLC7A11 and GPx4. These results suggest that KL may be involved in D-gal-induced H9C2 cellular aging during ferroptosis, mainly thr

Author

Xicheng Xiong; Gang Wang; Yiping Wang; Tian Zhang; Yali Bao; Kun Wang; Dina Ainiwaer; Zhan Sun

Publisher

Elsevier BV

Published

2023

Language

EN