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Sensitivity improvement by using contactless conductivity rather than indirect UV detection for the determination of enantiomeric purity of amines by CE by Marie Lecoeur-Lorin; Raphaël Delépée; Philippe Morin is a Engineering article available to read on EtoBox.

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## Abstract A capacitively coupled contactless conductivity detection (C4D) system for CE with a flexible detection cell was applied for the enantioseparation of small chiral underivatized amines using chiral crown ether or CD as chiral selector. Since these compounds are poorly UV‐active, C4D was an alternative detection mode. The composition (ionic strength, pH, chiral selector) of the electrolyte was optimized in order to be suitable for C4D. (−)‐(18‐crown‐6)‐2,3,11,12‐tetracarboxylic acid was required as chiral selector to resolve the enantiomers of small polar amines. However, trimethyl‐β‐CD was suitable to separate amines possessing hydrophobic carbon chains. The performance of C4D was compared with indirect UV detection in terms of sensitivity, repeatability and accuracy. The linearity range of C4D was very large (1.5–1600 μg/mL) compared with the indirect UV linearity range (25–400 μg/mL) and allowed the determination of the enantiomeric purity of isopinocampheylamine up to 0.25%. The CE–C4D method has been fully validated by applying a novel strategy using accuracy profiles. All relative biases of the developed method were included within the ±15% limits of acceptance. C4D

Who reads Sensitivity improvement by using contactless conductivity rather than indirect UV detection for the determination of enantiomeric purity of amines by CE?

Author

Marie Lecoeur-Lorin; Raphaël Delépée; Philippe Morin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2009

Language

EN

Field

Engineering (Physical Sciences)