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Crystallization of Small Proteins Assisted by Green Fluorescent Protein by Nobuhiro Suzuki; Masahiko Hiraki; Yusuke Yamada; Naohiro Matsugaki; Noriyuki Igarashi; Ryuichi Kato; Ivan Dikic; David Drew; So Iwata; Soichi Wakatsuki; Masato Kawasaki is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Crystallization of Small Proteins Assisted by Green Fluorescent Protein about?
The generation of crystal lattice contacts by proteinaceous tags fused to target proteins is an attractive approach to aid in the crystallization of otherwise intractable proteins. Here, the use of green fluorescent protein (GFP) fusions for this purpose is demonstrated, using ubiquitin and the ubiquitinbinding motif (UBM) of Y-family polymerase as examples. The structure of the GFP-ubiquitin fusion protein revealed that the crystal lattice was formed by GFP moieties. Ubiquitin was accommodated in the lattice through interactions with the peripheral loops of GFP. However, in the GFP-UBM fusion crystal UBM formed extensive interactions with GFP and these interactions, together with UBM dimerization, mediated the crystal packing. Interestingly, the tyrosine residues that are involved in mediating crystal contacts in both GFP-ubiquitin and GFP-UBM crystals are arranged in a belt on the surface of the -barrel structure of GFP. Therefore, it is likely that GFP can assist in the crystallization of small proteins and of protein domains in general.
Who reads Crystallization of Small Proteins Assisted by Green Fluorescent Protein?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nobuhiro Suzuki; Masahiko Hiraki; Yusuke Yamada; Naohiro Matsugaki; Noriyuki Igarashi; Ryuichi Kato; Ivan Dikic; David Drew; So Iwata; Soichi Wakatsuki; Masato Kawasaki
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)