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Influence of size distribution of proteins, thiol and disulfide content in whole wheat flour on rheological and chapati texture of Indian wheat varieties by B.T. Manu; U.J.S. Prasada Rao is a scholarly article available to read on EtoBox.
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The influence of protein composition, as measured by size-exclusion high performance liquid chromatography (SE-HPLC), on rheological properties and chapati texture was investigated in the whole wheat flours of eight Indian wheat cultivars grown at a single location. Proteins were extracted using two-step procedure: extraction with buffer containing 0.5% SDS (SDS buffer), followed by sonication. The results showed that SDS buffer extracted 72-90% of the total flour protein in different varieties and 7-11% protein was extracted from the remaining residues by sonication. The proteins extracted were fractionated by SE-HPLC into large polymeric proteins (>130kDa), small polymeric proteins (80-130kDa) and monomeric proteins (10-80kDa). Total polymeric protein content in the flour protein showed a significant positive correlation with dough hardness (r=0.71, p<0.05) and positive correlation with chapati texture (r=0.58, p<0.05). Of the SDS extractable polymeric proteins, large polymeric protein in flour protein had significant positive correlation to dough hardness (r=0.89, p<0.05) and chapati cutting force, which reflects the chapati texture (r=0.70, p<0.05). Protein disulfide content sh
- Author
- B.T. Manu; U.J.S. Prasada Rao
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)
- Published
- 2008
- Language
- EN