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Determination of Fifteen Nucleotides in Cultured Human Mononuclear Blood and Umbilical Vein Endothelial Cells by Solvent Generated Ion-pair Chromatography by M Cichna; M Raab; H Daxecker; A Griesmacher; M.M Müller; P Markl is a Chemistry article available to read on EtoBox.
What is Determination of Fifteen Nucleotides in Cultured Human Mononuclear Blood and Umbilical Vein Endothelial Cells by Solvent Generated Ion-pair Chromatography about?
The paper describes the development of a method for the determination of 15 nucleotides in cultured mononuclear blood and umbilical vein endothelial cell lysates by solvent generated ion-pair chromatography. The phase system is generated via a mobile phase of 100 mM phosphoric acid adjusted to pH 6.2 with triethylamine. Nucleotides are eluted by applying a linear magnesium ion gradient. The method is robust, highly reproducible and easily adaptable to other cell lysates and allows the separation and quantitation of the nucleotides with detection limits in the range from 17 (ADP) to 126 (CDP) pmol in 20-microl aliquots.
Who reads Determination of Fifteen Nucleotides in Cultured Human Mononuclear Blood and Umbilical Vein Endothelial Cells by Solvent Generated Ion-pair Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- M Cichna; M Raab; H Daxecker; A Griesmacher; M.M Müller; P Markl
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)
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