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**Very short patch repair: reducing the cost of cytosine methylation** by Margaret Lieb; Ashok S. Bhagwat is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is **Very short patch repair: reducing the cost of cytosine methylation** about?

In __Escherichia coli__ and related bacteria, the product of gene __dcm__ methylates the second cytosine of 5′‐CCWGG sequences (where W is A or T). Deamination of 5‐methylcytosine (5meC) results in C to T mutations. The mutagenic potential of 5meC is reduced by a system called very short patch (VSP) repair, which replaces T with C. T:G and U:G mispairs in the methylatable sequence and in related sequences are recognized by the product of __vsr__, a gene adjacent to __dcm__. Vsr creates a nick just 5′ of the mispaired pyrimidine to initiate the repair. Additional products known to be required for VSP repair are DNA polymerase I and DNA ligase. MutS and MutL have a stimulatory role but are not required. The ability of Vsr to recognize T:G mispairs in sequences related to CCWGG is probably responsible for over‐ and under‐representation of certain tetranucleotides in the __E. coli__ genome. Although VSP repair reduces spontaneous mutations at 5meCs in replicating bacteria, mutation hot‐spots persist at these sites. Under conditions that more accurately mimic the natural environment of __E. coli__, VSP repair appears to be effective in preventing mutation at 5meC.

Who reads **Very short patch repair: reducing the cost of cytosine methylation**?

Author

Margaret Lieb; Ashok S. Bhagwat

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)