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In Situ Identification and Visualization of Neurons That Mediate Enteric and Enteropancreatic Reflexes by Kirchgessner, Annette L.; Liu, Min-Tsai; Gershon, Michael D. is a Neuroscience article available to read on EtoBox.

What is In Situ Identification and Visualization of Neurons That Mediate Enteric and Enteropancreatic Reflexes about?

To identify neurons participating in enteric and enteropancreatic reflexes, we validated the use of the activity-dependent markers FM1-43 and FM2-10 as "on-line" probes for the visualization of activated guinea pig enteric and pancreatic neurons. FM1-43 or FM2-10 labeling of neuronal perikarya and processes was induced by KC1 (70 mM), veratridine (1.0 pM), intracellular injection of depolarizing current pulses, stimulation of afferent inputs, evoking reflexes (by inflating an intraluminal balloon, blowing puffs of Nz at, or applying glucose to, the villous surface of the duodenum), or injury; labeling was prevented by tetrodotoxin (0.5 pM). Intracellular recording and injection of Neurobiotinm confirmed that FM1-43 labeled neurons that spike, but not those that exhibit only fast excitatory postsynaptic potentials. Perikarya did not label if axonal transport was blocked by colchicine. When pulses of N2 or glucose were directed at duodenal villi in vitro, labeling by FM1-43 or FM2-10 was observed in myenteric and pancreatic neurons, as well as in subsets of cells in pancreatic islets and intestinal crypts. Hexamethonium blocked the spread of label via nicotinic synapses and thus enab

Who reads In Situ Identification and Visualization of Neurons That Mediate Enteric and Enteropancreatic Reflexes?

Author

Kirchgessner, Annette L.; Liu, Min-Tsai; Gershon, Michael D.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)