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Vaspin promotes chondrogenic differentiation of BMSCs via Akt activation in osteoarthritis by Junfeng Wang; Keshi Zhang; Shaolong Zhang; Zhenpeng Guan is a Medicine article available to read on EtoBox.

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## Abstract ## Background The aim of this study was to investigate the role of Vaspin on the chondrogenic differentiation of bone mesenchymal stem cells (BMSCs), and its effect on chondrocyte survival and ECM secretion. We also assessed whether the Akt activation participates in these processes. ## Methods In vivo, immunohistochemistry was used to examine the positive rate of the protein expressions of Akt in Wistar rat articular cartilage and subchondral bone after Vaspin intraperitoneal injection for 14 days. In vitro, we isolated and expanded BMSCs from Wistar rats, and further cultured BMSCs as pellets in a chondrogenic-differentiation medium supplemented with different concentrations of Vaspin. After 21 days, the pellets were processed for cell counting kit assay. The mRNA level of Akt, SOX9 and COL2A1 in the pellets were investigated using quantitative Real-Time polymerase chain reaction, and the protein level of COMP was detected using western blot. ## Results During the chondrogenic differentiation of BMSCs, Vaspin promoted the chondrogenic differentiation of BMSCs and chondrocyte survival by activating the Akt pathway. These effects were significantly reduced by treatment

Who reads Vaspin promotes chondrogenic differentiation of BMSCs via Akt activation in osteoarthritis?

Author

Junfeng Wang; Keshi Zhang; Shaolong Zhang; Zhenpeng Guan

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Medicine (Health Sciences)