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Adsorption of microorganisms onto an artificial siderophore-modified Au substrate by Tomohiko Inomata; Hiroshi Eguchi; Kenji Matsumoto; Yasuhiro Funahashi; Tomohiro Ozawa; Hideki Masuda is a scholarly article available to read on EtoBox.

What is Adsorption of microorganisms onto an artificial siderophore-modified Au substrate about?

The hydroxamate-type artificial siderophore, tris[2-{3-(N-acetyl-N-hydroxamino)propylamido}propyl]aminomethane (TAPPA) and its Fe III complex, Fe III -TAPPA were prepared and characterized by several spectroscopic methods. Fe III -TAPPA exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, Microbacterium flavescens, suggesting that Fe III -TAPPA can permeate the cell membrane of the microorganism. The adsorption of the Fe III -siderophore complex onto a deposited Au substrate was achieved by a stepwise self-assembling method. The modification of Fe III -TAPPA on the surface was confirmed from the cyclic voltammogram of the resultant Au electrode, Fe III -TAPPA/Au. The adsorption experiments of M. flavescens with Fe III -TAPPA/Au were monitored by optical, scanning electron, and atomic force microscopy and quartz crystal microbalance (QCM) measurements. These results clearly indicate that Fe III -TAPPA/Au can immobilize M. flavescens. This adsorption characteristic is due to the interaction between Fe III -TAPPA on an Au electrode and a receptor/binding protein within the cell membrane.

Author

Tomohiko Inomata; Hiroshi Eguchi; Kenji Matsumoto; Yasuhiro Funahashi; Tomohiro Ozawa; Hideki Masuda

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2007

Language

EN