About this Medicine article

Stability of Human Cytomegalovirus Genotypes in Persistently Infected Renal Transplant Recipients by Richard Stanton; Diana Westmoreland; Julie D. Fox; Andrew J. Davison; Gavin W.G. Wilkinson is a Medicine article available to read on EtoBox.

## Abstract Although most genes are highly conserved among strains of human cytomegalovirus (HCMV), several are unusually variable. By analyzing the sequence of two variable genes (__UL146__ and __UL74__) amplified directly from whole blood DNA extracts, multiple HCMV strains were detected in blood samples from 5/11 virus‐infected renal transplant patients. These five patients were seronegative prior to transplantation and their likely acquisition of the virus was via the donated organ; HCMV‐positive immunocompetent donors may thus be capable of harboring and transmitting multiple virus genotypes. In sequential samples taken up to 140 days post transplant no mutations in either gene were detected from 9/10 patients, and in the remaining patient a single nucleotide change was detected in __UL146__, and none in __UL74__. All sequences grouped into previously defined genotypes, with the detection of multiple members of the __UL74__ group 5 genotype establishing this previously tentative genotype. Additionally, identical sequences were identified in viruses from different patients, including examples from geographically distinct regions. Thus, although __UL146__ and __UL74__ exhibit im

Author

Richard Stanton; Diana Westmoreland; Julie D. Fox; Andrew J. Davison; Gavin W.G. Wilkinson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

2004

Language

EN

Field

Medicine (Health Sciences)