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Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency by Ji-Hoon Lee; Seung W. Ryu; Nicolette A. Ender; Tanya T. Paull is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency about?

Loss of the ataxia-telangiectasia mutated (ATM) kinase causes cerebellum-specific neurodegeneration in humans. We previously demonstrated that deficiency in ATM activation via oxidative stress generates insoluble protein aggregates in human cells, reminiscent of protein dysfunction in common neurodegenerative disorders. Here, we show that this process is driven by poly-ADP-ribose polymerases (PARPs) and that the insoluble protein species arise from intrinsically disordered proteins associating with PAR-associated genomic sites in ATM-deficient cells. The lesions implicated in this process are single-strand DNA breaks dependent on reactive oxygen species, transcription, and R-loops. Human cells expressing Mre11 A-T-like disorder mutants also show PARP-dependent aggregation identical to ATM deficiency. Lastly, analysis of A-T patient cerebellum samples shows widespread protein aggregation as well as loss of proteins known to be critical in human spinocerebellar ataxias that is not observed in neocortex tissues. These results provide a hypothesis accounting for loss of protein integrity and cerebellum function in A-T.

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Author

Ji-Hoon Lee; Seung W. Ryu; Nicolette A. Ender; Tanya T. Paull

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)