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Ecdysone agonist-inducible expression of a coat protein gene from tobacco mosaic virus confers viral resistance in transgenic Arabidopsis by Ja Choon Koo; Sebastian Asurmendi; Jennifer Bick; Terry Woodford-Thomas; Roger N. Beachy is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary Constitutive expression of a gene encoding tobacco mosaic virus (TMV) coat protein (CP) in transgenic plants confers resistance to infection by TMV and related tobamoviruses. Here, we examined resistance to TMV by temporal and quantitative control of TMV Cg CP (__CgCP__) gene expression using a simple, methoxyfenozide‐inducible system in __Arabidopsis__ plants. By soil drenching with a commercial ecdysone agonist (Intrepid‐2F/methoxyfenozide), most transgenic lines were induced from undetectable levels of gene expression to protein levels from 0.05 to 0.8% (w/w) of CgCP. This corresponds to up to four times the amount of CP produced by the constitutive cauliflower mosaic virus (CaMV) double 35S promoter. CgCP transcripts were induced by 700‐fold, without changing the expression patterns of pathogenesis‐related (PR) genes. The high level of accumulation of CgCP was sufficient to produce large amounts of virus‐like particles that accumulate in large aggregates throughout the cells. In virus challenge assays, treatment with Intrepid‐2F prior to TMV infection resulted in high levels of viral resistance, while no treatment or treatment with the inducer following infection did

Who reads Ecdysone agonist-inducible expression of a coat protein gene from tobacco mosaic virus confers viral resistance in transgenic Arabidopsis?

Author

Ja Choon Koo; Sebastian Asurmendi; Jennifer Bick; Terry Woodford-Thomas; Roger N. Beachy

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2004

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)