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What is Biotin Content Determination Method about?
1. This document provides a method validation report for the determination of biotin content in raw materials using HPLC. 2. The method involves extracting biotin from samples using an alkaline solution, diluting the extract, and then analyzing via HPLC with UV detection. 3. Key steps include preparing standard solutions of biotin, extracting samples by shaking and sonicating in NaOH and water, filtering the extract, and injecting it into the HPLC for analysis.
- Author
- Khanh Tuan
- Language
- EN