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Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures by Danny Chee Furng Wong; Niki Soo Ching Wong; John Soo Yang Goh; Lee May May; Miranda Gek Sim Yap is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures about?
## Abstract One of the goals of recombinant glycoprotein production is to achieve consistent glycosylation. Although many studies have examined the changes in the glycosylation quality of recombinant protein with culture, very little has been done to examine the underlying changes in glycosylation gene expression as a culture progresses. In this study, the expression of 24 genes involved in __N__‐glycosylation were examined using quantitative RT PCR to gain a better understanding of recombinant glycoprotein glycosylation during production processes. Profiling of the __N__‐glycosylation genes as well as concurrent analysis of glycoprotein quality was performed across the exponential, stationary and death phases of a fed‐batch culture of a CHO cell line producing recombinant human interferon‐γ (IFN‐γ). Of the 24 __N__‐glycosylation genes examined, 21 showed significant up‐ or down‐regulation of gene expression as the fed‐batch culture progressed from exponential, stationary and death phase. As the fed‐batch culture progressed, there was also an increase in less sialylated IFN‐γ glycoforms, leading to a 30% decrease in the molar ratio of sialic acid to recombinant IFN‐γ. This correlat
Who reads Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Danny Chee Furng Wong; Niki Soo Ching Wong; John Soo Yang Goh; Lee May May; Miranda Gek Sim Yap
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)