About this document
BiotechnologyTechniques12183 1861998 by Arshia Nazir is a document available to read on EtoBox.
This document evaluates the use of Tween 80 as a substrate for measuring lipolytic activity in enzymes. It establishes a spectrophotometric assay for Tween 80 hydrolysis and compares the activity of various lipases and esterases with Tween 80 against conventional substrates. The findings indicate that Tween 80 exhibits similar activity to p-nitrophenyl butyrate, positioning it between esterase and lipase categories.
- Author
- Arshia Nazir
- Language
- EN