Can I read Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction on EtoBox?

Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction by Ute Moreth; Olaf Schmidt is a scholarly article available to read on EtoBox.

What is Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction about?

## Summary The internal transcribed spacer (ITS) of the nuclear ribosomal DNA (rDNA) of the main fungal species causing wood rot damages in European buildings was amplified by the polymerase chain reaction (PCR). After sequencing the ITS, fungus-specific oligonucleotide primers were designed for taxon-specific priming PCR. These DNA marker molecules were suitable for the differential diagnosis of the Dry rot fungus, __Serpula lacrymans__, the Wild merulius, __S. himantioides__, the Oak polypore, __Donkioporia expansa__, the Brown cellar fungus, __Coniophora puteana__, the Broad-spored white polypore, __Antrodia vaillantii__, the Sap polypore, __Tyromyces placenta__, and the Yellow-red gill polypore, __Gloeophyllum sepiarium__. Each specific marker identified isolates of its respective target species. Cross reaction with ‘foreign’ fungi was the exception. Species detection from rot samples in buildings was possible, since DNA from contaminating organisms does not response to the marker molecules. The diagnosis was rapid, since preceding fungal pure cultures, special DNA extraction/purification and restriction by endonucleases are not required.

Author

Ute Moreth; Olaf Schmidt

Publisher

Walter de Gruyter GmbH

Published

2000

Language

EN