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Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography by Hua Wang; Yuhang Yang; Jing Guo; Mengli Wang; Hongfen Zhang; Guangbin Zhang; Ruimiao Chang; Anjia Chen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography about?
## Introduction: Picfeltarraenins ia, ib and iv and acteoside are the four bioactive ingredients of picria fel-terrae lour. their pharmacological effects include central inhibitory, cardiovascular, anti-inflammatory, anti-pyretic, analgesic, anti-bacterial, antioxidative and anti-tumor effects. ## Objective: We aimed to develop an efficient micellar electrokinetic chromatography (mekc) method modified with mixed organic solvents for the simultaneous separation and determination of the four components in picriae herba and its formulations. ## Methods: Method optimization was carried out by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of the four components in picriae herba and its formulations. ## Results: The optimal running buffer was composed of 20 mm sodium tetraborate, 40 mm sodium cholate, 10% (v/v) methanol and 10% (v/v) isopropanol (ph 9.76). the separation voltage was 18 kv, the temperature was 25°c and the detection wavelength was 266 nm. under the optimal separation conditions, the baseline separation of four components was achieved in less than 14 min. the correlation coefficients of the
Who reads Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hua Wang; Yuhang Yang; Jing Guo; Mengli Wang; Hongfen Zhang; Guangbin Zhang; Ruimiao Chang; Anjia Chen
- Publisher
- Wiley
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)