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Can I read Pipecolic Acid Hydroxylases: A Monophyletic Clade among cis ‐Selective Bacterial Proline Hydroxylases that Discriminates l‐Proline on EtoBox?
Pipecolic Acid Hydroxylases: A Monophyletic Clade among cis ‐Selective Bacterial Proline Hydroxylases that Discriminates l‐Proline by Johanna Mattay; Wolfgang Hüttel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Pipecolic Acid Hydroxylases: A Monophyletic Clade among cis ‐Selective Bacterial Proline Hydroxylases that Discriminates l‐Proline about?
## Abstract Proline hydroxylases are iron(II)/2‐oxoglutarate‐dependent enzymes that hydroxylate l‐proline and derivatives, such as lpipecolic acid, which is the six‐membered‐ring homologue of l‐proline. It has been established that there is a distinct group of conserved bacterial enzymes that hydroxylate l‐pipecolic acid and __trans__‐3‐ and __trans__‐4‐methyl‐l‐proline, but virtually no l‐proline. This allows the organism to produce hydroxyproline congeners without hydroxylation of the physiologically omnipresent l‐proline. In vitro conversions showed that the substrate spectrum of the pipecolic acid hydroxylases GetF (from a __Streptomyces__ sp.; producer of the tetrapeptide antibiotic GE81112) and PiFa (from __Frankia alni__) overlaps that of proline hydroxylases, except for the nonacceptance of l‐proline and smaller homologues. Distinct and conserved residues were determined for both types of enzymes. However, site‐directed mutagenesis in GetF did not yield variants that accepted l‐proline; this suggested a complex interaction of several residues around the active site, which resulted in delicate changes in substrate specificity. This is supported by substrate docking in a homo
Who reads Pipecolic Acid Hydroxylases: A Monophyletic Clade among cis ‐Selective Bacterial Proline Hydroxylases that Discriminates l‐Proline?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Johanna Mattay; Wolfgang Hüttel
- Publisher
- Wiley
- Published
- 2017
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)