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In vivo effects of endotoxin on DNA synthesis in rat nasal epithelium by Dr. J. R. Harkema; J. A. Hotchkiss is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Airway inflammation in bacterial infections is characterized by the presence of neutrophils and often epithelial injury and repair. Release of endotoxin from bacteria may contribute to these processes. The purpose of this study was to determine the in vivo effects of repeated endotoxin exposure on DNA synthesis in rat nasal epithelium in the presence and absence of neutrophilic influx. Rats were intranally instilled, once a day for 3 days, with endotoxin or saline (controls). Before the first and third instillations, half of the saline and endotoxin‐instilled animals were depleted of circulating blood neutrophils by administering a rabbit anti‐rat neutrophil antiserum. Rats were sacrificed 6 or 24 h after the last instillation. Two hours prior to sacrifice, rats were intraperitoneally injected with bromodeoxyuridine (BrdU), an analog of thymidine that is incorporated in the nucleus of cells in the S‐phase of the cell cycle. Nasal tissues were processed for light microscopy and immunohistochemical detection of BrdU in nasal epithelial cells. The numbers of nasal epithelial cells, BrdU‐labeled epithelial nuclei, and neutrophils per millimeter of basal lamina in the epithe

Who reads In vivo effects of endotoxin on DNA synthesis in rat nasal epithelium?

Author

Dr. J. R. Harkema; J. A. Hotchkiss

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)