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Enhancing the production of gamma-linolenic acid in Hansenula polymorpha by fed-batch fermentation using response surface methodology by Bhimabol Khongto; Kobkul Laoteng; Anan Tongta is a Chemistry article available to read on EtoBox.

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## Abstract Gamma-linolenic acid (GLA, C18:3Δ6,9,12) is an n-6 polyunsaturated fatty acid (PUFA) that has been used for the alleviation and treatment of a number of symptoms and diseases. Increasing GLA demand has led to a search for alternative producers and potential strategies for GLA production. Based on the successful performance of Hansenula polymorpha, a methylotrophic yeast, as a “cell factory” for the production of valuable bioproducts, a bioprocess development approach was implemented for GLA production in the recombinant yeast carrying the mutated Δ6-desaturase gene of Mucor rouxii. Using a substrate-feeding strategy under glycerol-limited conditions, the physical-chemical variables during the fed-batch fermentation of the recombinant H. polymorpha were optimised for GLA production through response surface methodology using a Box-Behnken design. The medium composition, including yeast extract and trace elements, and dissolved oxygen tension (DOT) were targeted. We found that DOT was the most effective variable for enhancing GLA yield. These results also suggest that the optimum conditions for GLA production are 28 % saturation of DOT, 1 g L−1 of yeast extract and 3.6 mL

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Author

Bhimabol Khongto; Kobkul Laoteng; Anan Tongta

Publisher

Versita; Springer-Verlag; Springer International Publishing AG; Bratislava: Institute of Chemistry Slovak Academy of Sciences; Warszawa: Versita; Heidelberg: Springer, 2006-2014.; Bratislava: Institute of Chemistry Slovak Academy of Sciences; Berlin: De Gruyter, 2015-2016.; Bratislava: Institute of Chemistry Slovak Academy of Sciences; Heidelberg: Springer, 2017-; Bratislava: Institute of Chemistry Slovak Academy of Sciences; Warszawa: De Gruyter, 2016-2016; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0366-6352)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)