Skip to content

Opening book details…

Can I read Data on Optimization of Microprojectile Bombardment Parameters in Development of Salinity Tolerant Transgenic Lines on EtoBox?

Data on Optimization of Microprojectile Bombardment Parameters in Development of Salinity Tolerant Transgenic Lines by Sarangi, Susmita; Mandal, Chiranjib; Dutta, Sourav; Mukherjee, Pranit; Mondal, Raju; Kumar, S.P. Jeevan; Choudhury, P. Ray; Singh, Vijay Pratap; Tripathi, Durgesh Kumar; Mandal, Asit B. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Data on Optimization of Microprojectile Bombardment Parameters in Development of Salinity Tolerant Transgenic Lines about?

This data deals with the optimization of microprojectile bombardment particles for efficient genetic transformation in an __indica__ rice involving __AmSOD__ gene for development of salinity tolerant transgenic lines [1]. In this study, various parameters such as effect of genotypes, helium pressure, osmoticum, explants, flight distance, particle size, particle volume, vacuum, carrier DNA and stopping screen properties have been evaluated to determine their role in transformation of __indica__ rice involving __AmSOD__ gene for development of salinity tolerant Pusa Basmati 1 rice variety. To perform the transformation process, plasmid vector pCAMBIA 1305.2 was used, which harbours GUS PlusTM gene, intron from the castor bean catalase gene, pBR322 ori, kanamycin resistant gene and __Xho__ I site. The transformants have been confirmed using slot blot, polymerase chain reaction and Southern hybridization techniques.

Who reads Data on Optimization of Microprojectile Bombardment Parameters in Development of Salinity Tolerant Transgenic Lines?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Sarangi, Susmita; Mandal, Chiranjib; Dutta, Sourav; Mukherjee, Pranit; Mondal, Raju; Kumar, S.P. Jeevan; Choudhury, P. Ray; Singh, Vijay Pratap; Tripathi, Durgesh Kumar; Mandal, Asit B.
Publisher
Elsevier ; Elsevier BV; New York: Elsevier Inc. (ISSN 2352-3409)
Published
2020
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Physical Sciences)

More by Sarangi, Susmita; Mandal, Chiranjib; Dutta, Sourav; Mukherjee, Pranit; Mondal, Raju; Kumar, S.P. Jeevan; Choudhury, P. Ray; Singh, Vijay Pratap; Tripathi, Durgesh Kumar; Mandal, Asit B.

Browse all works by Sarangi, Susmita; Mandal, Chiranjib; Dutta, Sourav; Mukherjee, Pranit; Mondal, Raju; Kumar, S.P. Jeevan; Choudhury, P. Ray; Singh, Vijay Pratap; Tripathi, Durgesh Kumar; Mandal, Asit B.