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Diagnosis of HIV‐1 infection by detection of antibody IgG to HIV‐1 in urine with ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens by Seiichi Hashida; Kazuya Hashinaka; Atsushi Saitoh; Akihisa Takamizawa; Hideo Shinagawa; Shinichi Oka; Kaoru Shimada; Kouichi Hirota; Takeyuki Kohno; Setsuko Ishikawa; Dr. Eiji Ishikawa is a Medicine article available to read on EtoBox.

What is Diagnosis of HIV‐1 infection by detection of antibody IgG to HIV‐1 in urine with ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens about?

Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT), p i 7 and p24 as antigens, and p-o-galactosidase from Escherichia coli as label. Anti-HIV-I IgG in urine was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-recombinant protein conjugate and recombinant prOtein-P-D-galaCtOSidaSe conjugate. The immune complex formed, consisting of the three components, was trapped onto polystyrene balls coated with affinitypurified (anti-2,4-dinitrophenyl group) IgG. After washing, the immune complex was eluted from the polystyrene balls with excess of &N-2,4-dinitrophenyl-~-lysine and transferred to clean polystyrene balls coated with aff inity-purified (anti-human IgG ychain) IgG. Finally, the enzyme activity bound to the last solid phase was assayed by fluorometry. Using recombinant RT as antigen, the sensitivity and specificity for 83 seropositives and 100 seronegatives were both 1 OO%, and the lowest signal for 60 asymptomatic carriers was 8.2-fold higher than the highest signal for the seronegatives. The positivity with recombinant RT as antigen could be confi

Who reads Diagnosis of HIV‐1 infection by detection of antibody IgG to HIV‐1 in urine with ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens?

Author

Seiichi Hashida; Kazuya Hashinaka; Atsushi Saitoh; Akihisa Takamizawa; Hideo Shinagawa; Shinichi Oka; Kaoru Shimada; Kouichi Hirota; Takeyuki Kohno; Setsuko Ishikawa; Dr. Eiji Ishikawa

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-8013)

Published

1994

Language

EN

Field

Medicine (Health Sciences)