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Metabolic Glycan Labeling-Based Screen to Identify Bacterial Glycosylation Genes by Moulton, Karen D.; Adewale, Adedunmola P.; Carol, Hallie A.; Mikami, Sage A.; Dube, Danielle H. is a Medicine article available to read on EtoBox.

What is Metabolic Glycan Labeling-Based Screen to Identify Bacterial Glycosylation Genes about?

Bacterial cell surface glycans are quintessential drug targets due to their critical role in colonization of the host, pathogen survival, and immune evasion. The dense cell envelope glycocalyx contains distinctive monosaccharides that are stitched together into higher order glycans to yield exclusively bacterial structures that are critical for strain fitness and pathogenesis. However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging. Bacteria produce glycans containing rare sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the identification of their biosynthesis machinery. To ease the study of bacterial glycans in the absence of detailed structural information, we used metabolic glycan labeling to detect changes in glycan biosynthesis. Here, we screened wild-type versus mutant strains of the gastric pathogen , ultimately permitting the identification of genes involved in glycoprotein and lipopolysaccharide biosynthesis. Our findings provide the first evidence that protein glycosylation proceeds via a lipid carrier-mediated pathway that overlaps with lipopolysaccharide biosynthesis. Protein g

Who reads Metabolic Glycan Labeling-Based Screen to Identify Bacterial Glycosylation Genes?

Author

Moulton, Karen D.; Adewale, Adedunmola P.; Carol, Hallie A.; Mikami, Sage A.; Dube, Danielle H.

Publisher

American Chemical Society; American Chemical Society (ACS) (ISSN 2373-8227)

Published

2020

Language

EN

Field

Medicine (Life Sciences)