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Methanol production during chemotaxis to amino acids in Bacillus subtilis by John R. Kirby; Christopher J. Kristich; Shelby L. Feinberg; George W. Ordal is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Methanol production during chemotaxis to amino acids in Bacillus subtilis about?

The 20 common amino acids act as attractants during chemotaxis by the Gram‐positive organism __Bacillus subtilis__. In this study, we report that all amino acids induce __B. subtilis__ to produce methanol both upon addition and removal of the chemoeffector. Asparagine‐induced methanol production is specific to the McpB receptor and aspartate‐induced methanol production correlates with receptor occupancy. These findings suggest that addition and removal of all amino acids cause demethylation of specific receptors which results in methanol production. We also demonstrate that certain attractants cause greater production of methanol after multiple stimulations. CheC and CheD, while affecting the levels of receptor methylation, are not absolutely required for either methylation or demethylation. In contrast, CheY is necessary for methanol formation upon removal of attractant but not upon addition of attractant. We conclude that methanol formation due to negative stimuli indicates the existence of a unique adaptational mechanism in __B. subtilis__ involving the response regulator, CheY.

Who reads Methanol production during chemotaxis to amino acids in Bacillus subtilis?

Author

John R. Kirby; Christopher J. Kristich; Shelby L. Feinberg; George W. Ordal

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)