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Genetic alteration and PD-L1 expression profiles of Chinese patients with lung squamous cell carcinoma 1 1 Introduction 1 2 Materials and methods 2 2.1 Biospecimen collection and clinical data 2 2.2 DNA isolation 2 2.3 Target next-generation sequencing 2 2.4 Data processing 2 2.5 Germline alteration interpretation 3 2.6 Actionable alteration annotation 3 2.7 PD-L1 immunohistochemistry 3 2.8 Statistical analysis 3 3 Results 4 3.1 Patient characteristics 4 3.2 Mutational feature in driver genes and comprehensive feature 4 3.3 Differences in the prevalence of driver genes between Chinese and western cohort 5 3.4 Germline variants 5 3.5 Comparison of the genetic findings between tissue and plasma samples 5 3.6 Clinically actionable alterations 6 3.7 Relationship between somatic alternations and PD-L1 expression 6 4 Discussion 6 5 Conclusions 8 Ethics approval and consent to participate 8 Consent for publication 8 Funding 8 CRediT authorship contribution statement 8 Competing interests 8 Data Availability 9 Acknowledgments 9 Appendix A Supplementary material 9 References 9

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Language

UND

Category

nonfiction