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Plasmon-coupled microcavity aptasensors for visual and ultra-sensitive simultaneous detection of Staphylococcus aureus and Escherichia coli by Milan Lei; Chunxiang Xu; Yaqi Shan; Chuansheng Xia; Ru Wang; Huan-Huan Ran; Fu-Gen Wu; Ruipeng Chen; Xiangwei Zhao; Qiannan Cui is a Chemistry article available to read on EtoBox.
What is Plasmon-coupled microcavity aptasensors for visual and ultra-sensitive simultaneous detection of Staphylococcus aureus and Escherichia coli about?
Septicemia and bacteremia are serious infections in the bloodstream. Thus, time-saving and ultra-sensitive pathogenic bacteria detection is highly required. Herein, we constructed gold nanoparticle-modified polystyrene microspheres (Au/PS) as plasmoncoupled microcavities to realize simultaneous detection of Staphylococcus aureus and Escherichia coli based on a fluorescence and surface-enhanced Raman spectroscopy (SERS) dual-mode method. Fluorescence imaging, serving as a means for assistant validation and rapid screening, was carried out to achieve qualitative and semi-quantitative determination, which gave us visual information of the existence and distribution of the target bacteria. Meanwhile, SERS test was conducted to realize ultra-sensitive quantitative detection. The evanescent wave aroused from total internal reflection in PS microcavities coupled with the localized electromagnetic field from surface plasmons of gold nanoparticles to improve light-matter interaction synergistically, leading to an enhancement factor of 2.25 × 10 11 for SERS sensing. The whole measurement was carried out in a typical sandwich assay of "capture probe-target bacteria-signal probe." As a result,
Who reads Plasmon-coupled microcavity aptasensors for visual and ultra-sensitive simultaneous detection of Staphylococcus aureus and Escherichia coli?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Milan Lei; Chunxiang Xu; Yaqi Shan; Chuansheng Xia; Ru Wang; Huan-Huan Ran; Fu-Gen Wu; Ruipeng Chen; Xiangwei Zhao; Qiannan Cui
- Publisher
- Springer Science and Business Media LLC
- Published
- 2020
- Language
- EN
- Field
- Chemistry (Physical Sciences)