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Diagnostic ions for the rapid analysis by nano‐electrospray ionization quadrupole time‐of‐flight mass spectrometry of O ‐glycans from human mucins by Catherine Robbe; Calliope Capon; Bernadette Coddeville; Jean‐Claude Michalski is a Chemistry article available to read on EtoBox.

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## Abstract Nano‐electrospray ionization quadrupole time‐of‐flight mass spectrometry (nanoESI‐Q‐TOFMS) was used for sensitive mapping and sequencing of underivatized oligosaccharide alditols obtained from human mucins. Using subnanomolar amounts of oligosaccharides previously analyzed by nuclear magnetic resonance (NMR), series of diagnostic ions relevant to the structural characterization of __O__‐glycans were deduced. Determination of the core type as well as positions and partial linkages of fucose residues could be readily obtained from the dominant [M+Na]^+^ ions. Differentiation of isomeric structures and glycosidic linkages were defined by the characteristic cross‐ring ^0,2^A‐type cleavages in the negative ion mode. Tandem (MS/MS) mass spectra of [MH]^−^ ions from sialylated or sulfated __O__‐glycans revealed information concerning the position and linkage of such residues. These fragmentation rules were further applied in the structural determination of glycans from human colonic mucins. All these findings indicated the efficiency of ESI‐Q‐TOFMS for the determination of oligosaccharide composition, sequence, partial linkage and substitution, providing a wealth of structura

Who reads Diagnostic ions for the rapid analysis by nano‐electrospray ionization quadrupole time‐of‐flight mass spectrometry of O ‐glycans from human mucins?

Author

Catherine Robbe; Calliope Capon; Bernadette Coddeville; Jean‐Claude Michalski

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2004

Language

EN

Field

Chemistry (Physical Sciences)