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What is SDS-PAGE Gel Preparation Recipes about?

This document provides recipes and instructions for performing SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) to separate proteins. It includes recipes for a 12.5% running gel and 4% stacking gel, as well as stock solutions including 30% acrylamide, 1.5M Tris pH 8.8, 0.5M Tris pH 6.8, 10% ammonium persulfate, SDS running buffer, and 2X SDS sample buffer. The procedure involves adding reagents except TEMED to the gels, filtering, degassing, then adding TEMED and pouring the gels.

Author

Sky Imran

Language

EN