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Environmental Applications of Nucleic Acid Amplification Technology by Gary A. Toranzos is a book available to read on EtoBox.

What is Environmental Applications of Nucleic Acid Amplification Technology about?

<p><P>From the Preface Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been used successfully for a variety of samples, but this method was still not sensitive enough. The next logical step was the application of the recently developed DNA amplification technique known as the polymerase chain reaction, or PCR. Since then, many laboratories around the world have adopted PCR for environmental work. <br>Samples obtained from soils, water and air are enormously complex because they are unknown mixtures of DNA and other compounds. Thus, procedures for target DNA amplification from the environment require special attention. The PCR has allowed us to go beyond the need for culturing prior to analysis of microbial communities. It has been shown that even microorganisms that can be routinely grown in the laboratory undergo some physiological changes when exposed to the environment. One of these changes (first observe

Author

Gary A. Toranzos

Publisher

Technomic Publishing Company, Incorporated

Published

1997

Language

EN

ISBN

9781566764087

Subjects

Engineering, Mathematics, Technology